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TH (phospho Ser19) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60863

  • Amount:

    100ul

  • Molecular Weight:

    58524 Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Tyrosine Hydroxylase around the phosphorylation site of Ser19. AA range:10-59

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-TH Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    TH

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Tyrosine 3-monooxygenase

  • Human Gene Id:

    7054

  • Human Swiss Prot No:

    P07101

  • Mouse Gene Id:

    21823

  • Mouse Swiss Prot No:

    P24529

  • Rat Gene Id:

    25085

  • Rat Swiss Prot No:

    P04177

  • Specificity:

    Phospho-TH (S19) Polyclonal Antibody detects endogenous levels of TH protein only when phosphorylated at S19.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH

  • Observed Band(KD):

    45

  • Function:

    catalytic activity:L-tyrosine + tetrahydrobiopterin + O(2) = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.,cofactor:Fe(2+) ion.,disease:Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.,enzyme regulation:Phosphorylation leads to an increase in the catalytic activity.,function:Plays an important role in the physiology of adrenergic neurons.,online information:Tyrosine hydroxylase entry,pathway:Ca

  • Subcellular Location:

    Cytoplasm, perinuclear region . Nucleus . Cell projection, axon . Cytoplasm . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle . When phosphorylated at Ser-19 shows a nuclear distribution and when phosphorylated at Ser-31 as well at Ser-40 shows a cytosolic distribution (By similarity). Expressed in dopaminergic axons and axon terminals. .

  • Expression:

    Mainly expressed in the brain and adrenal glands.

  • Western Blot analysis of 293 cells using Phospho-TH Polyclonal Antibody diluted at 1:1000

  • Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 30', using Tyrosine Hydroxylase Antibody. The lane on the right is blocked with the phospho peptide.

  • mmunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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Catalog Number

  • Amount:100ul
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TH (phospho Ser19) Polyclonal Antibody | EnoGene Biotech Co. Ltd