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Cytokeratin 18 (phospho Ser33) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60859

  • Amount:

    100ul

  • Molecular Weight:

    47927 Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33. AA range:1-50

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Cytokeratin 18 Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    KRT18

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Keratin type I cytoskeletal 18

  • Human Gene Id:

    3875

  • Human Swiss Prot No:

    P05783

  • Mouse Gene Id:

    16668

  • Mouse Swiss Prot No:

    P05784

  • Rat Gene Id:

    294853

  • Rat Swiss Prot No:

    Q5BJY9

  • Specificity:

    Phospho-Cytokeratin 18 (S33) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S33.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    KRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

  • Observed Band(KD):

    47

  • Function:

    disease:Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].,function:Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.,induction:By IL-6.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.,PTM:Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either

  • Subcellular Location:

    Cytoplasm, perinuclear region. Nucleus, nucleolus.

  • Expression:

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • Western blot analysis of lysates from HT29 cells, using Keratin 18 Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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Cytokeratin 18 (phospho Ser33) Polyclonal Antibody | EnoGene Biotech Co. Ltd