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IκB-ε (phospho Ser22) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60858

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22. AA range:131-180

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    NFKBIE

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Background:

    NFKB inhibitor epsilon(NFKBIE) Homo sapiens The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011],

  • Protein Name:

    NF-kappa-B inhibitor epsilon

  • Human Gene Id:

    4794

  • Human Swiss Prot No:

    O00221

  • Mouse Gene Id:

    18037

  • Mouse Swiss Prot No:

    O54910

  • Specificity:

    Phospho-IκB-ε (S22) Polyclonal Antibody detects endogenous levels of IκB-ε protein only when phosphorylated at S22.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NFKBIE; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon

  • MolecularWeight(Da):

    38448

  • Observed Band(KD):

    38

  • Function:

    function:Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.,PTM:Serine phosphorylated; followed by proteasome-dependent degradation.,similarity:Belongs to the NF-kappa-B inhibitor family.,similarity:Contains 6 ANK repeats.,subunit:Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.,tissue specificity:Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.,

  • Subcellular Location:

    cytoplasm,Golgi apparatus,cytosol,perinuclear region of cytoplasm,

  • Expression:

    B-cell,Brain,Fetal brain,Pancreas,Synovial membrane,Synovial membrane tissue,

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IkappaB-epsilon (Phospho-Ser22) Antibody

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Catalog Number

  • Amount:100ul
  • Price:225$

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IκB-ε (phospho Ser22) Polyclonal Antibody | EnoGene Biotech Co. Ltd