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HSP90β (phospho Ser254) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60857

  • Amount:

    100ul

  • Molecular Weight:

    83133 Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-HSP90β Polyclonal Antibody

  • Gene Name:

    HSP90AB1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],

  • Protein Name:

    Heat shock protein HSP 90-beta

  • Human Gene Id:

    3326

  • Human Swiss Prot No:

    P08238

  • Mouse Gene Id:

    15516

  • Mouse Swiss Prot No:

    P11499

  • Rat Gene Id:

    301252

  • Rat Swiss Prot No:

    P34058

  • Specificity:

    Phospho-HSP90β (S254) Polyclonal Antibody detects endogenous levels of HSP90β protein only when phosphorylated at S254.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84

  • Observed Band(KD):

    90

  • Function:

    function:Molecular chaperone. Has ATPase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 90 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with TP53/p53 (By similarity). Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer.,

  • Subcellular Location:

    Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673). Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762). .

  • Expression:

    Amygdala,Brain cortex,Colon,Colon carcinoma,Embryon

  • Western Blot analysis of HepG2 cells using Phospho-HSP90β Polyclonal Antibody

  • Enzyme-Linked Immunosorbent Assay for Immunogen Phosphopeptide and Non-Phosphopeptide, using HSP90B Antibody

  • Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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HSP90β (phospho Ser254) Polyclonal Antibody | EnoGene Biotech Co. Ltd