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Adducin α/β (phospho Ser726/713) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60854

  • Amount:

    100ul

  • Molecular Weight:

    80955 Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726. AA range:688-737

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Adducin α/β Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    ADD1/ADD2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Alpha-adducin/Beta-adducin

  • Human Gene Id:

    118/119

  • Human Swiss Prot No:

    P35611/P35612

  • Mouse Gene Id:

    11518/11519

  • Rat Gene Id:

    24170/24171

  • Rat Swiss Prot No:

    Q63028/Q05764

  • Specificity:

    Phospho-Adducin α/β (S726/713) Polyclonal Antibody detects endogenous levels of Adducin α/β protein only when phosphorylated at S726/713.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta

  • Observed Band(KD):

    80

  • Function:

    alternative products:Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.,

  • Subcellular Location:

    Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

  • Expression:

    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

  • Western blot analysis of lysates from HeLa cells treated with Forskolin 40nM 30', using ADD1 Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

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Adducin α/β (phospho Ser726/713) Polyclonal Antibody | EnoGene Biotech Co. Ltd