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14-3-3 ζ (phospho Ser58) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60853

  • Amount:

    100ul

  • Molecular Weight:

    27745 Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-14-3-3 ζPolyclonal Antibody diluted at 1:2000

  • Gene Name:

    YWHAZ

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

  • Protein Name:

    14-3-3 protein zeta/delta

  • Human Gene Id:

    7534

  • Human Swiss Prot No:

    P63104

  • Mouse Gene Id:

    22631

  • Mouse Swiss Prot No:

    P63101

  • Rat Gene Id:

    25578

  • Rat Swiss Prot No:

    P63102

  • Specificity:

    Phospho-14-3-3 ζ (S58) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein only when phosphorylated at S58.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

  • Observed Band(KD):

    30

  • Function:

    caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to

  • Subcellular Location:

    Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.

  • Expression:

    B-cell lymphoma,Bone marrow

  • Western Blot analysis of Jurkat cells using Phospho-14-3-3 ζ Polyclonal Antibody diluted at 1:2000

  • Enzyme-Linked Immunosorbent Assay for Immunogen Phosphopeptide and Non-Phosphopeptide, using 14-3-3 zeta Antibody

  • Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta Antibody. The lane on the right is blocked with the phospho peptide.

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  • Amount:100ul
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