DRP1 (phospho Ser637) Polyclonal Antibody

Name: DRP1 (phospho Ser637) Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-60841
Price: 225 USD
Availability: InStock

E20-60841Cite ID: EN0049767Datasheet COA MSDS

ReactivityHuman;Mouse;RatFields:>>Necroptosis;>>NOD-like receptor signaling pathway;>>TNF signaling pathway

ApplicationsWB;IHC-p;IF/ICC;ELISA

SourcePolyclonal, Rabbit,IgG

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight81877Da

Protein NameDynamin-1-like protein

Human Gene Id10059

Human Swiss Prot NoO00429

Mouse Gene Id74006

Mouse Swiss Prot NoQ8K1M6

Rat Gene Id114114

Rat Swiss Prot NoO35303

SpecificityPhospho-DRP1 (S637) Polyclonal Antibody detects endogenous levels of DRP1 protein only when phosphorylated at S637(human), S643(mouse), S656(rat), .

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameDNM1L; DLP1; DRP1; Dynamin-1-like protein; Dnm1p/Vps1p-like protein; DVLP; Dynamin family member proline-rich carboxyl-terminal domain less; Dymple; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; HdynIV; Dynamin-rela

Observed Band(KD)81

Functioncatalytic activity:GTP + H(2)O = GDP + phosphate.,function:Functions in mitochondrial and peroxisomal division probably by regulating membrane fission. Enzyme hydrolyzing GTP that oligomerizes to form ring-like structures and is able to remodel membranes. May also play a role on organelles of the secretory pathway.,miscellaneous:Isoform 1 and isoform 2 inhibits peroxisomal division when overexpressed while isoform 3 and isoform 4 have no effect.,PTM:Phosphorylated by GSK3B.,similarity:Belongs to the dynamin family.,similarity:Contains 1 GED domain.,subcellular location:Mainly cytosolic. Also membrane-associated. Localizes to mitochondria at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear.,subunit:Homotetramer; N-terminal part b

Subcellular LocationCytoplasm, cytosol. Golgi apparatus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane ; Peripheral membrane protein. Peroxisome. Membrane, clathrin-coated pit . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Mainly cytosolic. Recruited by RALA and RALBP1 to mitochondrion during mitosis (PubMed:21822277). Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Colocalized with MARCHF5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Localizes to mitochondria following necrosis induction. Recruited to the mitochondrial outer membrane by interaction with MIEF1. Mitochondrial recruitment is inhibited by C11orf65/MFI (By similarity). Associated with peroxisomal membranes, partly re

ExpressionUbiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenSynthesized phospho-peptide around the phosphorylation site of human DRP1 (phospho Ser637)

Gene NameDNM1L

DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

BackgroundThis gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013],

Western Blot analysis of JK cells using Phospho-DRP1 (S637) Polyclonal Antibody.

Immunohistochemical analysis of paraffin- embedded human tonsil. 1, Antibody was diluted at 1:200(4 ℃ overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-60841-Conjugate. Price: $485 / Size: 100μl.

E20-60841-FITC$485 / 100μlE20-60841-Biotin$485 / 100μlE20-60841-HRP$485 / 100μlE20-60841-AP$485 / 100μlE20-60841-AF350$485 / 100μlE20-60841-AF405M$485 / 100μlE20-60841-AF488$485 / 100μlE20-60841-AF514$485 / 100μlE20-60841-AF532$485 / 100μlE20-60841-AF555$485 / 100μlE20-60841-AF568$485 / 100μlE20-60841-AF405S$485 / 100μlE20-60841-AF405L$485 / 100μlE20-60841-AF546$485 / 100μlE20-60841-AF594$485 / 100μlE20-60841-AF610$485 / 100μlE20-60841-AF635$485 / 100μlE20-60841-AF647$485 / 100μlE20-60841-AF680$485 / 100μlE20-60841-AF700$485 / 100μlE20-60841-AF750$485 / 100μlE20-60841-AF790$485 / 100μlE20-60841-APC$485 / 100μlE20-60841-PE$485 / 100μlE20-60841-Cy3$485 / 100μlE20-60841-Cy5$485 / 100μlE20-60841-Cy5.5$485 / 100μlE20-60841-Cy7$485 / 100μlE20-60841-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.