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PC-PLD2 (phospho Tyr169) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60829

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PLD2 around the phosphorylation site of Tyr169. AA range:136-185

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PLD2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLD2 (Phospho-Tyr169) Antibody

  • Background:

    phospholipase D2(PLD2) Homo sapiens The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011],

  • Protein Name:

    Phospholipase D2

  • Human Gene Id:

    5338

  • Human Swiss Prot No:

    O14939

  • Mouse Gene Id:

    18806

  • Mouse Swiss Prot No:

    P97813

  • Rat Gene Id:

    25097

  • Rat Swiss Prot No:

    P70498

  • Specificity:

    Phospho-PC-PLD2 (Y169) Polyclonal Antibody detects endogenous levels of PC-PLD2 protein only when phosphorylated at Y169.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PLD2; Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2

  • MolecularWeight(Da):

    105987

  • Observed Band(KD):

    95

  • Function:

    catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,enzyme regulation:Stimulated by phosphatidylinositol 4,5-bisphosphate and activated by the ADP-ribosylation factor-1 (ARF-1).,function:May have a role in signal-induced cytoskeletal regulation and/or endocytosis.,online information:Phospholipase D entry,similarity:Belongs to the phospholipase D family.,similarity:Contains 1 PH domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 PLD phosphodiesterase domains.,subunit:Interacts with EGFR (By similarity). Interacts with PIP5K1A.,tissue specificity:Ubiquitous.,

  • Subcellular Location:

    intracellular,endoplasmic reticulum membrane,Golgi apparatus,plasma membrane,presynapse,

  • Expression:

    B-cell,Brain,Skin,Uterus,

  • Immunohistochemistry analysis of paraffin-embedded human brain, using PLD2 (Phospho-Tyr169) Antibody. The picture on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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