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PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60765

  • Amount:

    100ul

  • Molecular Weight:

    83598 Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat;Chicken(testedbyourcustomer）

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607. AA range:573-622

  • Source:

    Polyclonal, Rabbit,IgG

  • Lu, Xueying, et al. "Luteolin induces apoptosis in vitro through suppressing the MAPK and PI3K signaling pathways in gastric cancer." Oncology letters 14.2 (2017): 1993-2000.

  • Gene Name:

    PIK3R1

  • Dilution:

    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],

  • Protein Name:

    Phosphatidylinositol 3-kinase regulatory subunit alpha

  • Human Gene Id:

    5295

  • Human Swiss Prot No:

    P27986

  • Mouse Gene Id:

    18708

  • Mouse Swiss Prot No:

    P26450

  • Rat Gene Id:

    25513

  • Rat Swiss Prot No:

    Q63787

  • Specificity:

    Phospho-PI 3-kinase p85α (Y607) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α protein only when phosphorylated at Y607.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

  • Observed Band(KD):

    80

  • Function:

    disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat

  • Subcellular Location:

    nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,

  • Expression:

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

  • Western Blot analysis of various cells using Phospho-PI 3-kinase p85α Polyclonal Antibody diluted at 1:1000

  • Western Blot analysis of KB cells using Phospho-PI 3-kinase p85α Polyclonal Antibody diluted at 1:1000

  • Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody | EnoGene Biotech Co. Ltd