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Rad52 (phospho Tyr104) Polyclonal Antibody
Datasheet
Catalog Number:
E20-60758Amount:
100ulSwiss-Prot No.:
P43351/ P43352Molecular Weight:
46169，Observed Band : 46kdApplications:
WB,IHC-p,IF(paraffin section),ELISAReactivity:
Human,Mouse,MonkeyPurification:
The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.Immunogen:
Phosphopeptide (Phospho-left) and Non Phosphopeptide (Phospho-right), using RAD52 (PhosphoTyr104) AntibodySpecificity/Sensitivity:
Phospho-Rad52 (Y104) Polyclonal Antibody detects endogenous levels of
Rad52 protein only when phosphorylated at Y104.Storage/Stability:
-20°C/1 yearSource:
RabbitGene Name:
RAD52Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA:
1/20000. Not yet tested in other applications.
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for
Background:
RAD52 homolog, DNA repair protein(RAD52) Homo sapiens The protein
encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a
protein important for DNA double-strand break repair and homologous
recombination. This gene product was shown to bind single-stranded DNA ends,
and mediate the DNA-DNA interaction necessary for the annealing of
complementary DNA strands. It was also found to interact with DNA
recombination protein RAD51, which suggested its role in RAD51 related DNA
recombination and repair. A pseudogene of this gene is present on chromosome
2. Alternative splicing results in multiple transcript variants. Additional
alternatively spliced transcript variants of this gene have been described, but their
full-length nature is not known. [provided by RefSeq, Jul 2014],Gene ID:
5893/ 19365Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azideFunction:
Involved in double-stranded break repair. Plays a central role in geneticrecombination and DNA repair by promoting the annealing of complementary
single-stranded DNA and by stimulation of the RAD51
recombinase.,PTM:Phosphorylated upon DNA damage, probably by ATM or
ATR.,similarity:Belongs to the RAD52 family.,subunit:Forms a undecameric ring.
- Immunohistochemistry analysis of paraffin-embedded human
testis, using RAD52 (Phospho-Tyr104) Antibody. The picture on
the right is blocked with the phospho peptide. 
- Western blot analysis of lysates from COS7 cells treated with
H2O2 100uM 30', using RAD52 (Phospho-Tyr104) Antibody. The
lane on the right is blocked with the phospho peptide.
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