You are here: EnoGene Home > Products > Cell Signal Transduction Research Antibodies > PAKα (phospho Ser199) Polyclonal Antibody

PAKα (phospho Ser199) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60757

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PAK1 around the phosphorylation site of Ser199. AA range:165-214

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PAK1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Immunohistochemistry analysis of paraffin-embedded human placenta, using PAK1 (Phospho-Ser199) Antibody. The picture on the right is blocked with the phospho peptide.

  • Background:

    p21 (RAC1) activated kinase 1(PAK1) Homo sapiens This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],

  • Protein Name:

    Serine/threonine-protein kinase PAK 1

  • Human Gene Id:

    5058

  • Human Swiss Prot No:

    Q13153

  • Mouse Swiss Prot No:

    O88643

  • Rat Gene Id:

    29431

  • Rat Swiss Prot No:

    P35465

  • Specificity:

    Phospho-PAKα (S199) Polyclonal Antibody detects endogenous levels of PAKα protein only when phosphorylated at S199.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PAK1; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK

  • MolecularWeight(Da):

    60647

  • Observed Band(KD):

    61

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-423 and allows the kinase domain to adopt an active structure. Also activated by binding to GTP-bound CDC42, independent of the phosphorylation state of Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation.,function:The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apop

  • Subcellular Location:

    ruffle,cytoplasm,Golgi apparatus,cytosol,plasma membrane,cell-cell junction,focal adhesion,intercalated disc,Z disc,axon,dendrite,filamentous actin,nuclear membrane,ruffle membrane,protei

  • Expression:

    Epithelium,Pituitary tumor,Placenta,

  • Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.

Add to Cart

Related Products

PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    145$
  • Add to Cart
PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    225$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    145$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    225$
  • Add to Cart
Rel (Phospho-Ser503) Antibody
Rel (Phospho-Ser503) Antibody Datasheet  

  • Catalog Number:

    E011020-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human

  • Applications:

    IF:1:100~1:200

  • Swiss-Prot No.:

    Q04864

  • Price:

    145$
  • Add to Cart

Catalog Number

  • Amount:100ul
  • Price:225$

Add to Cart

AbPromise

Contact us

PAKα (phospho Ser199) Polyclonal Antibody | EnoGene Biotech Co. Ltd