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Smad3 (phospho Thr179) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60745

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179. AA range:145-194

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of HELA cells using Phospho-Smad3 (T179) Polyclonal Antibody

  • Gene Name:

    SMAD3

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    SMAD family member 3(SMAD3) Homo sapiens The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

  • Protein Name:

    Mothers against decapentaplegic homolog 3

  • Human Gene Id:

    4088

  • Human Swiss Prot No:

    P84022

  • Mouse Gene Id:

    17127

  • Mouse Swiss Prot No:

    Q8BUN5

  • Rat Gene Id:

    25631

  • Rat Swiss Prot No:

    P84025

  • Specificity:

    Phospho-Smad3 (T179) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at T179.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

  • MolecularWeight(Da):

    48081

  • Observed Band(KD):

    50

  • Function:

    disease:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].,domain:The MH2 domain is sufficient to carry protein nuclear export.,function:Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with Smad4.,subunit:Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts wit

  • Subcellular Location:

    nuclear chromatin,intracellular,nucleus,nuclear inner membrane,nucleoplasm,transcription factor complex,cytoplasm,cytosol,plasma membrane,receptor complex,SMAD protein complex,SMAD2-SMAD3 protein complex,

  • Expression:

    Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood

  • Immunohistochemistry analysis of paraffin-embedded human heart, using Smad3 (Phospho-Thr179) Antibody. The picture on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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