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Cell Signal Transduction Research Antibodies
Cdk7 (phospho Thr170) Polyclonal Antibody
Human;Mouse
WB;IHC-p;IF(paraffin section);ELISA
Polyclonal, Rabbit,IgG
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight(Da):39038
Protein NameCyclin-dependent kinase 7
Human Gene Id1022
Human Swiss Prot NoP50613
Mouse Gene Id12572
Mouse Swiss Prot NoQ03147
SpecificityPhospho-Cdk7 (T170) Polyclonal Antibody detects endogenous levels of Cdk7 protein only when phosphorylated at T170.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCDK7; CAK; CAK1; CDKN7; MO15; STK1; Cyclin-dependent kinase 7; 39 kDa protein kinase; p39 Mo15; CDK-activating kinase 1; Cell division protein kinase 7; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subu
Observed Band(KD)40
Functioncatalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Inactivated by phosphorylation.,function:Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of
Subcellular LocationNucleus . Cytoplasm . Cytoplasm, perinuclear region . Colocalizes with PRKCI in the cytoplasm and nucleus (PubMed:15695176). Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (PubMed:19071173). .
ExpressionUbiquitous.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human CDK7 around the phosphorylation site of Thr170. AA range:136-185
Gene NameCDK7
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundcyclin dependent kinase 7(CDK7) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-60731-Conjugate. Price: $485 / Size: 100μl.
E20-60731-FITC$485 / 100μlE20-60731-Biotin$485 / 100μlE20-60731-HRP$485 / 100μlE20-60731-AP$485 / 100μlE20-60731-AF350$485 / 100μlE20-60731-AF405M$485 / 100μlE20-60731-AF488$485 / 100μlE20-60731-AF514$485 / 100μlE20-60731-AF532$485 / 100μlE20-60731-AF555$485 / 100μlE20-60731-AF568$485 / 100μlE20-60731-AF405S$485 / 100μlE20-60731-AF405L$485 / 100μlE20-60731-AF546$485 / 100μlE20-60731-AF594$485 / 100μlE20-60731-AF610$485 / 100μlE20-60731-AF635$485 / 100μlE20-60731-AF647$485 / 100μlE20-60731-AF680$485 / 100μlE20-60731-AF700$485 / 100μlE20-60731-AF750$485 / 100μlE20-60731-AF790$485 / 100μlE20-60731-APC$485 / 100μlE20-60731-PE$485 / 100μlE20-60731-Cy3$485 / 100μlE20-60731-Cy5$485 / 100μlE20-60731-Cy5.5$485 / 100μlE20-60731-Cy7$485 / 100μlE20-60731-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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