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PFK-2 car (phospho Ser483) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60711

  • Amount:

    100ul

  • Molecular Weight:

    58477 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483. AA range:451-500

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-PFK-2 car Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    PFKFB2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

  • Human Gene Id:

    5208

  • Human Swiss Prot No:

    O60825

  • Mouse Gene Id:

    18640

  • Mouse Swiss Prot No:

    P70265

  • Rat Gene Id:

    24640

  • Rat Swiss Prot No:

    Q9JJH5

  • Specificity:

    Phospho-PFK-2 car (S483) Polyclonal Antibody detects endogenous levels of PFK-2 car protein only when phosphorylated at S483.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PFKFB2; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme

  • Observed Band(KD):

    58

  • Function:

    catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in the activation of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Heart.,

  • Subcellular Location:

    cytosol,

  • Expression:

    Heart.

  • Western Blot analysis of Hela cells using Phospho-PFK-2 car Polyclonal Antibody diluted at 1:1000

  • Enzyme-Linked Immunosorbent Assay for Immunogen Phosphopeptide and Non-Phosphopeptide, using PFKFB2 Antibody

  • Western blot analysis of lysates from 293 cells treated with Heat shock, using PFKFB2 Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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PFK-2 car (phospho Ser483) Polyclonal Antibody | EnoGene Biotech Co. Ltd