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PKC θ (phospho Thr538) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60705

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538. AA range:504-553

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PRKCQ

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Background:

    protein kinase C theta(PRKCQ) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Protein kinase C theta type

  • Human Gene Id:

    5588

  • Human Swiss Prot No:

    Q04759

  • Specificity:

    Phospho-PKC θ (T538) Polyclonal Antibody detects endogenous levels of PKC θ protein only when phosphorylated at T538.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta

  • MolecularWeight(Da):

    81865

  • Observed Band(KD):

    81

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activ

  • Subcellular Location:

    iimmunological synapse,intracellular,cytosol,plasma membrane,

  • Expression:

    Blood,Brain,

  • Immunohistochemistry analysis of paraffin-embedded human brain, using PKC thet (Phospho-Thr538) Antibody. The picture on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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PKC θ (phospho Thr538) Polyclonal Antibody | EnoGene Biotech Co. Ltd