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Raf-1 (phospho Ser296) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60690

  • Amount:

    100ul

  • Swiss-Prot No.:

    P04049/ Q99N57/ P11345

  • Molecular Weight:

    73052，Observed Band : 74kd

  • Applications:

    WB,IHC-p,IF(paraffin section),ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.

  • Immunogen:

    Phosphopeptide (Phospho-left) and NonPhosphopeptide (Phospho-right), using C-RAF (PhosphoSer296) Antibody

  • Specificity/Sensitivity:

    Phospho-Raf-1 (S296) Polyclonal Antibody detects endogenous levels of Raf-1
    protein only when phosphorylated at S296.

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Western Blot analysis of 293 cells using Phospho-Raf-1 (S296)
    Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    RAF1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA:
    1/20000. Not yet tested in other applications

  • Background:

    Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene
    is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP
    kinase kinase kinase (MAP3K), which functions downstream of the Ras family of
    membrane associated GTPases to which it binds directly. Once activated, the
    cellular RAF1 protein can phosphorylate to activate the dual specificity protein
    kinases MEK1 and MEK2, which in turn phosphorylate to activate the
    serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are
    pleiotropic effectors of cell physiology and play an important role in the control of
    gene expression involved in the cell division cycle, apoptosis, cell differentiation
    and cell migration. Mutations in this gene are associated with Noonan syndrome 5
    and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],

  • Gene ID:

    5894/110157/ 24703

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2
    zinc ions per subunit.,disease:Defects in RAF1 are the cause of LEOPARD
    syndrome type 2 (LEOPARD syndrome-2) [MIM:611554]. LEOPARD syndrome is
    an autosomal dominant disorder allelic with Noonan syndrome. The acronym
    LEOPARD stands for lentigines, electrocardiographic conduction abnormalities,
    ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of
    growth, and deafness.,disease:Defects in RAF1 are the cause of Noonan
    syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder
    characterized by dysmorphic facial features, short stature, hypertelorism, cardiac
    anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically
    heterogeneous and relatively common syndrome, with an estimated incidence of
    1 in 1000-2500 live births.,function:Involved in the transducti

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for

  • Immunohistochemistry analysis of paraffin-embedded human
    breast carcinoma, using C-RAF (Phospho-Ser296) Antibody. The
    picture on the right is blocked with the phospho peptide.

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Raf-1 (phospho Ser296) Polyclonal Antibody | EnoGene Biotech Co. Ltd