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FAK (phospho Tyr407) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60649

  • Amount:

    100ul

  • Molecular Weight:

    119233 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr407. AA range:373-422

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-FAK Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    PTK2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    protein tyrosine kinase 2(PTK2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015],

  • Protein Name:

    Focal adhesion kinase 1

  • Human Gene Id:

    5747

  • Human Swiss Prot No:

    Q05397

  • Mouse Gene Id:

    14083

  • Mouse Swiss Prot No:

    P34152

  • Rat Gene Id:

    25614

  • Rat Swiss Prot No:

    O35346

  • Specificity:

    Phospho-FAK (Y407) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at Y407.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

  • Observed Band(KD):

    125

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,domain:The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.,domain:The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.,function:Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.,PTM:Phosphorylated on 6 tyrosine residues upon activatio

  • Subcellular Location:

    Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules.

  • Expression:

    Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Expressed in epithelial cells (at protein level) (PubMed:31630787).

  • Western Blot analysis of 293T cells using Phospho-FAK Polyclonal Antibody diluted at 1:1000

  • Immunohistochemistry analysis of paraffin-embedded human brain, using FAK Antibody. The picture on the right is blocked with the phospho peptide.

  • Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using FAK Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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FAK (phospho Tyr407) Polyclonal Antibody | EnoGene Biotech Co. Ltd