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c-Fos (phospho Ser362) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60631

  • Amount:

    100ul

  • Molecular Weight:

    40695 Da

  • Applications:

    WB;CoIP;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Fos around the phosphorylation site of Ser362. AA range:331-380

  • Source:

    Polyclonal, Rabbit,IgG

  • Li, X., et al. "ORF45-mediated prolonged." (2015).

  • Gene Name:

    FOS

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Proto-oncogene c-Fos

  • Human Gene Id:

    2353

  • Human Swiss Prot No:

    P01100

  • Mouse Gene Id:

    14281

  • Mouse Swiss Prot No:

    P01101

  • Rat Gene Id:

    140675

  • Rat Swiss Prot No:

    P12841

  • Specificity:

    Phospho-c-Fos (S362) Polyclonal Antibody detects endogenous levels of c-Fos protein only when phosphorylated at S362.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7

  • Observed Band(KD):

    62

  • Function:

    function:Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.,PTM:Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.,PTM:Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK

  • Subcellular Location:

    Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.

  • Expression:

    Lung adenocarcinoma,Pancreas,Tongue,

  • Western Blot analysis of various cells using Phospho-c-Fos Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

  • Western Blot analysis of 293T cells using Phospho-c-Fos Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

  • Western blot analysis of lysates from K562 cells treated with forskolin 40nM 30', using Fos Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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c-Fos (phospho Ser362) Polyclonal Antibody | EnoGene Biotech Co. Ltd