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Syk (phospho Tyr348) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60614

  • Amount:

    100ul

  • Molecular Weight:

    72066 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human SYK around the phosphorylation site of Tyr348. AA range:314-363

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Syk Polyclonal Antibody

  • Gene Name:

    SYK

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],

  • Protein Name:

    Tyrosine-protein kinase SYK

  • Human Gene Id:

    6850

  • Human Swiss Prot No:

    P43405

  • Mouse Gene Id:

    20963

  • Mouse Swiss Prot No:

    P48025

  • Rat Gene Id:

    25155

  • Rat Swiss Prot No:

    Q64725

  • Specificity:

    Phospho-Syk (Y348) Polyclonal Antibody detects endogenous levels of Syk protein only when phosphorylated at Y348.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk

  • Observed Band(KD):

    72

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.,PTM:Autophosphorylated.,PTM:Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling.,PTM:Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phas

  • Subcellular Location:

    Cell membrane . Cytoplasm, cytosol .

  • Expression:

    Widely expressed in hematopoietic cells (at protein level) (PubMed:8163536). Expressed in neutrophils (at protein level) (PubMed:15123770). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells (PubMed:8163536).

  • Western Blot analysis of 3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody

  • Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using SYK Antibody. The lane on the right is blocked with the phospho peptide.

  • Western blot analysis of lysates from 1) 3T3, 2) HT29, 3) HepG2 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibodywas diluted at 1:10000, 37° 1hour.

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Catalog Number

  • Amount:100ul
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Syk (phospho Tyr348) Polyclonal Antibody | EnoGene Biotech Co. Ltd