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Stat5 (phospho Ser726/731) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60613

  • Amount:

    100ul

  • Molecular Weight:

    89866 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human STAT5B around the phosphorylation site of Ser731. AA range:697-746

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Stat5 Polyclonal Antibody diluted at 1:500

  • Gene Name:

    STAT5A/STAT5B

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been

  • Protein Name:

    Signal transducer and activator of transcription 5A/B

  • Human Gene Id:

    6777

  • Human Swiss Prot No:

    P42229/P51692

  • Mouse Gene Id:

    20850/20851

  • Rat Gene Id:

    24918/25126

  • Rat Swiss Prot No:

    Q62771/P52632

  • Specificity:

    Phospho-Stat5 (S726/731) Polyclonal Antibody detects endogenous levels of Stat5 protein only when phosphorylated at S726/731.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B

  • Observed Band(KD):

    90

  • Function:

    function:Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.,online information:STAT5 entry,PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7.,

  • Subcellular Location:

    Cytoplasm . Nucleus . Translocated into the nucleus in response to phosphorylation.

  • Expression:

    Brain,Cervix,Epithelium,Lung,Placenta,Synovial memb

  • Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using STAT5B Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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Stat5 (phospho Ser726/731) Polyclonal Antibody | EnoGene Biotech Co. Ltd