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Lyn (phospho Tyr508) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60604

  • Amount:

    100ul

  • Molecular Weight:

    58443 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Lyn around the phosphorylation site of Tyr507. AA range:463-512

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Lyn Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    LYN

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011],

  • Protein Name:

    Tyrosine-protein kinase Lyn

  • Human Gene Id:

    4067

  • Human Swiss Prot No:

    P07948

  • Mouse Gene Id:

    17096

  • Mouse Swiss Prot No:

    P25911

  • Rat Gene Id:

    81515

  • Rat Swiss Prot No:

    Q07014

  • Specificity:

    Phospho-Lyn (Y508) Polyclonal Antibody detects endogenous levels of Lyn protein only when phosphorylated at Y508.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    LYN; JTK8; Tyrosine-protein kinase Lyn; Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn

  • Observed Band(KD):

    53,56

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with phosphorylated LIME1 and with CD79A upon BCR activation. Interacts with Epstein-Barr virus LMP2A. Interacts with TGFB1I1. Interaction, via the SH2 and SH3, domains with MUC1 is stimulated by IL7 and, the subsequent phosphorylation increases the binding between MUC1 and CTNNB1/beta-catenin. Interacts with PPP1R15A via the SH3 domain.,tissue specificity:Expressed in primary neuroblastoma tumors.,

  • Subcellular Location:

    Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Membrane ; Lipid-anchor . Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. Detected on plasma membrane lipid rafts.

  • Expression:

    Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.

  • Western Blot analysis of 293 cells using Phospho-Lyn Polyclonal Antibody diluted at 1:1000

  • Enzyme-Linked Immunosorbent Assay for Immunogen Phosphopeptide and Non-Phosphopeptide, using Lyn Antibody

  • Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using Lyn Antibody. The lane on the right is blocked with the phospho peptide.

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