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HSL (phospho Ser855) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60602
  • Amount:

    100ul
  • Price:

    225$
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554. AA range:520-569
  • Source:

    Polyclonal, Rabbit
  • Gene Name:

    LIPE
  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
  • Western blot analysis of L929 using p-HSL (S855) antibody. Antibody was diluted at 1:1000
  • Background:

    lipase E, hormone sensitive type(LIPE) Homo sapiens The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008],
  • Protein Name:

    Hormone-sensitive lipase
  • Human Gene Id:

    3991
  • Human Swiss Prot No:

    Q05469
  • Mouse Gene Id:

    16890
  • Mouse Swiss Prot No:

    P54310
  • Rat Gene Id:

    25330
  • Rat Swiss Prot No:

    P15304
  • Specificity:

    Phospho-HSL (S855) Polyclonal Antibody detects endogenous levels of HSL protein only when phosphorylated at S855.
  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Concentration:

    1 mg/ml
  • Storage Stability:

    -20°C/1 year
  • Other Name:

    LIPE; Hormone-sensitive lipase; HSL
  • MolecularWeight(Da):

    116568
  • Observed Band(KD):

    81
  • Function:

    catalytic activity:Diacylglycerol + H(2)O = monoacylglycerol + a carboxylate.,catalytic activity:Monoacylglycerol + H(2)O = glycerol + a carboxylate.,catalytic activity:Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.,enzyme regulation:Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.,function:In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.,pathway:Glycerolipid metabolism; triacylglycerol degradation.,similarity:Belongs to the 'GDXG' lipolytic enzyme family.,subcellular location:Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.,subunit:Interacts wi
  • Subcellular Location:

    lipid particle,cytosol,caveola,
  • Expression:

    Testis,
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSL (Phospho-Ser855/554) Antibody

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Catalog Number

  • Amount:100ul
  • Price:225$

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HSL (phospho Ser855) Polyclonal Antibody | EnoGene Biotech Co. Ltd