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eEF2K (phospho Ser366) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60599

  • Amount:

    100ul

  • Molecular Weight:

    82172 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. AA range:331-380

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-eEF2K Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    EEF2K

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Eukaryotic elongation factor 2 kinase

  • Human Gene Id:

    29904

  • Human Swiss Prot No:

    O00418

  • Mouse Swiss Prot No:

    O08796

  • Rat Gene Id:

    25435

  • Rat Swiss Prot No:

    P70531

  • Specificity:

    Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

  • Observed Band(KD):

    105

  • Function:

    catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer .,

  • Subcellular Location:

    cytoplasm,cytosol,postsynaptic density,

  • Expression:

    Epithelium,Glial tumor,Lymph,T-cell,

  • Western blot analysis of lysates from HeLa cells treated with serum 10% 15', using eEF2K Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

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eEF2K (phospho Ser366) Polyclonal Antibody | EnoGene Biotech Co. Ltd