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Caspase-9 (phospho Thr125) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60598

  • Amount:

    100ul

  • Molecular Weight:

    46281 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Thr125. AA range:91-140

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Caspase-9 Polyclonal Antibody diluted at 1:500

  • Gene Name:

    CASP9

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013],

  • Protein Name:

    Caspase9

  • Human Gene Id:

    842

  • Human Swiss Prot No:

    P55211

  • Rat Gene Id:

    58918

  • Rat Swiss Prot No:

    Q9JHK1

  • Specificity:

    Phospho-Caspase-9 (T125) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at T125.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

  • Observed Band(KD):

    46

  • Function:

    catalytic activity:Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).,function:Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.,online information:Caspase-9 entry,PTM:Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers

  • Subcellular Location:

    nucleus,mitochondrion,cytosol,apoptosome,

  • Expression:

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

  • Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5'+calyculinA 50ng/ml 5', using Caspase 9 Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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Caspase-9 (phospho Thr125) Polyclonal Antibody | EnoGene Biotech Co. Ltd