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C/EBP α (phospho Ser21) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60597

  • Amount:

    100ul

  • Molecular Weight:

    37575 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-C/EBP αPolyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

  • Gene Name:

    CEBPA

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],

  • Protein Name:

    CCAAT/enhancer-binding protein alpha

  • Human Gene Id:

    1050

  • Human Swiss Prot No:

    P49715

  • Mouse Gene Id:

    12606

  • Mouse Swiss Prot No:

    P53566

  • Rat Gene Id:

    24252

  • Rat Swiss Prot No:

    P05554

  • Specificity:

    Phospho-C/EBP α (S21) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at S21.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha

  • Observed Band(KD):

    45

  • Function:

    function:C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X.,

  • Subcellular Location:

    Nucleus .; [Isoform 4]: Nucleus, nucleolus .

  • Expression:

    Liver,Pancreas,Umbilical cord,White Matter pool- 5 brain tissues- f

  • Western Blot analysis of mouse liver, rat liver, hela cells, hela cell treated or untreated by insulin. Primary Antibody was diluted at 1:1000. Secondary antibody.

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Catalog Number

  • Amount:100ul
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C/EBP α (phospho Ser21) Polyclonal Antibody | EnoGene Biotech Co. Ltd