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ACCα (phospho Ser80) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60595

  • Amount:

    100ul

  • Molecular Weight:

    265554 Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Acetyl-CoA Carboxylase around the phosphorylation site of Ser80. AA range:46-95

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-ACCα Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    ACACA

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Acetyl-CoA carboxylase 1

  • Human Gene Id:

    31

  • Human Swiss Prot No:

    Q13085

  • Mouse Gene Id:

    107476

  • Mouse Swiss Prot No:

    Q5SWU9

  • Rat Gene Id:

    60581

  • Rat Swiss Prot No:

    P11497

  • Specificity:

    Phospho-ACCα (S80) Polyclonal Antibody detects endogenous levels of ACCα protein only when phosphorylated at S80.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

  • Observed Band(KD):

    280

  • Function:

    catalytic activity:ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA.,catalytic activity:ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.,cofactor:Binds 2 manganese ions per subunit.,cofactor:Biotin.,disease:Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.,enzyme regulation:By phosphorylation.,function:Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.,online information:Acetyl-CoA carboxylase entry,pathway:Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: st

  • Subcellular Location:

    Cytoplasm, cytosol .

  • Expression:

    Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

  • Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Acetyl-CoA Carboxylase Antibody. The lane on the right is blocked with the phospho peptide.

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ACCα (phospho Ser80) Polyclonal Antibody | EnoGene Biotech Co. Ltd