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EphA2/3/4 (phospho Tyr588/596) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60550

  • Amount:

    100ul

  • Molecular Weight:

    108254/110131/109860Da

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human EPHA2/3 around the phosphorylation site of Tyr588/596. AA range:556-605

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of HepG2 cells using Phospho-EphA2/3/4 Y588/596) Polyclonal Antibody

  • Gene Name:

    EPHA2/3/4

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Ephrin type-A receptor 2/3/4

  • Human Gene Id:

    1969/2042/2043

  • Human Swiss Prot No:

     P29317/P29320/P54764

  • Specificity:

    Phospho-EphA2/3/4 (Y588/596) Polyclonal Antibody detects endogenous levels of EphA2/3/4 protein only when phosphorylated at Y588/596.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EPHA2; ECK; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA3; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO

  • Observed Band(KD):

    130

  • Background:

    EPH receptor A2(EPHA2) Homo sapiens This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010],

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:Interacts with SLA (By similarity). Interacts with INPPL1/SHIP2.,tissue specificity:Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.,

  • Subcellular Location:

    intracellular,plasma membrane,integral component of plasma membrane,cell-cell adherens junction,focal adhesion,cell surface,lamellipodium,leading edge membrane,lamellipodium membrane,ruffle membrane,

  • Expression:

    Colon,Epithelium,Liver,Pancreas,

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using EPHA2/3 (Phospho-Tyr588/596) Antibody

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Catalog Number

  • Amount:100ul
  • Price:225$

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EphA2/3/4 (phospho Tyr588/596) Polyclonal Antibody | EnoGene Biotech Co. Ltd