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DAPK3 (phospho Thr265) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60548

  • Amount:

    100ul

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    . The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of HuvEc cells using Phospho-DAPK3 (T265) Polyclonal Antibody

  • Gene Name:

    DAPK3

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Death-associated protein kinase 3

  • Human Gene Id:

    1613

  • Human Swiss Prot No:

    O43293

  • Specificity:

    Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    DAPK3; ZIPK; Death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinaseMolecularWeight: 52536Da

  • Observed Band(KD):

    52

  • Background:

    death associated protein kinase 3(DAPK3) Homo sapiens Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.

  • Subcellular Location:

    nucleus,cytoplasm,actin filament,PML body,membrane raft

  • Immunofluorescence analysis of Hela cell. 1,DAPK3 (phospho Thr265) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-tubulin Monoclonal Antibody(8F11)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

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