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Brk (phospho Tyr447) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60537

  • Amount:

    100ug

  • Swiss-Prot No.:

    Q13882

  • Molecular Weight:

    51.8KD Observed Band: 50KD

  • Applications:

    WB,IF/ICC,ELISA

  • Reactivity:

    Human

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human BreastTumor Kinase around the phosphorylation site of Tyr447. AA range:402-451

  • Specificity/Sensitivity:

    Phospho-Brk (Y447) Polyclonal Antibody detects endogenous levels of Brk protein only when phosphorylated at Y447.

  • Other Names:

    PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Western Blot analysis of 293 cells using Phospho-Brk (Y447) Polyclonal Antibody

  • Gene Name:

    PTK6

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Gene ID:

    5753

  • Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.

  • Western blot analysis of lysates from 293 cells treatedwith EGF 200ng/ml 30', using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ug
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Brk (phospho Tyr447) Polyclonal Antibody | EnoGene Biotech Co. Ltd