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ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60497

  • Amount:

    100ul

  • Molecular Weight:

    (Da):41390

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized phospho-peptide around the phosphorylation site of human ERK 1/2 (phospho Tyr222/205)

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of KB cells using Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody diluted at 1:500

  • Gene Name:

    MAPK1/MAPK3

  • Dilution:

    IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300

  • Background:

    This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reporte

  • Protein Name:

    Mitogen-activated protein kinase 1

  • Human Gene Id:

    5594/5595

  • Human Swiss Prot No:

    P28482/P27361

  • Mouse Gene Id:

    26413/26417

  • Rat Gene Id:

    116590/50689

  • Rat Swiss Prot No:

    P63086/P21708

  • Specificity:

    Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y222/205.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK3; ER

  • Observed Band(KD):

    44

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by phosphorylation on tyrosine and threonine in response to insulin and NGF. Both phosphorylations are required for activity.,function:Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.,online information:Extracellular signal-regulated kinase entry,PTM:Dually phosphorylated on Thr-185 and Tyr-187, which activates the en

  • Subcellular Location:

    Cytoplasm, cytoskeleton, spindle . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm . Membrane, caveola . Cell junction, focal adhesion . Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization. .

  • Expression:

    Brain,Epithelium,Lung,Platelet,T-cell,

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  • Amount:100ul
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