You are here: EnoGene Home > Products > Cell Signal Transduction Research Antibodies > Raf-1 (phospho Ser301) Polyclonal Antibody

Raf-1 (phospho Ser301) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60435

  • Amount:

    100ul

  • Swiss-Prot No.:

    P04049/ Q99N57/P11345

  • Molecular Weight:

    73052，Observed Band : 75kd

  • Price:

    225$

  • Applications:

    WB, ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen

  • Immunogen:

    Synthesized phospho-peptide around the phosphorylation site of human Raf-1
    (phospho Ser301)

  • Specificity/Sensitivity:

    Phospho-Raf-1 (S301) Polyclonal Antibody detects endogenous levels of Raf-1
    protein only when phosphorylated at S301.

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Western Blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    RAF1

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other

  • Background:

    Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene
    is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP
    kinase kinase kinase (MAP3K), which functions downstream of the Ras family of
    membrane associated GTPases to which it binds directly. Once activated, the
    cellular RAF1 protein can phosphorylate to activate the dual specificity protein
    kinases MEK1 and MEK2, which in turn phosphorylate to activate the
    serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are
    pleiotropic effectors of cell physiology and play an important role in the control of
    gene expression involved in the cell division cycle, apoptosis, cell differentiation
    and cell migration. Mutations in this gene are associated with Noonan syndrome 5
    and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],

  • Gene ID:

    5894/110157/24703

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2zinc ions per subunit.,disease:Defects in RAF1 are the cause of LEOPARDsyndrome type 2 (LEOPARD syndrome-2) [MIM:611554]. LEOPARD syndrome isan autosomal dominant disorder allelic with Noonan syndrome. The acronymLEOPARD stands for lentigines, electrocardiographic conduction abnormalities,ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation ofgrowth, and deafness.,disease:Defects in RAF1 are the cause of Noonansyndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disordercharacterized by dysmorphic facial features, short stature, hypertelorism, cardiacanomalies, deafness, motor delay, and a bleeding diathesis. It is a geneticallyheterogeneous and relatively common syndrome, with an estimated incidence of1 in 1000-2500 live births.,function:Involved in the transducti

  • The picture was kindly provided by our customer,antibody was diluted at 1:500

Add to Cart

Related Products

PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    145$
  • Add to Cart
PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    225$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    145$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    225$
  • Add to Cart
Rel (Phospho-Ser503) Antibody
Rel (Phospho-Ser503) Antibody Datasheet  

  • Catalog Number:

    E011020-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human

  • Applications:

    IF:1:100~1:200

  • Swiss-Prot No.:

    Q04864

  • Price:

    145$
  • Add to Cart

Catalog Number

  • Amount:100ul
  • Price:225$

Add to Cart

AbPromise

Contact us