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MAK (phospho Tyr159) Polyclonal Antibody
Datasheet
Catalog Number:
E20-60423Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human MAK around the phosphorylation site of Tyr159. AA range:126-175Source:
Polyclonal, RabbitGene Name:
MAKDilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
- Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using MAK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.
Background:
male germ cell associated kinase(MAK) Homo sapiens The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],Protein Name:
Serine/threonine-protein kinase MAKHuman Gene Id:
4117Human Swiss Prot No:
P20794Mouse Gene Id:
17152Mouse Swiss Prot No:
Q04859Rat Gene Id:
25677Rat Swiss Prot No:
P20793Specificity:
Phospho-MAK (Y159) Polyclonal Antibody detects endogenous levels of MAK protein only when phosphorylated at Y159.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
MAK; Serine/threonine-protein kinase MAK; Male germ cell-associated kinaseMolecularWeight(Da):
70581Observed Band(KD):
85Function:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Could play an important function in spermatogenesis.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed mainly in testicular cells at and after meiosis.,Subcellular Location:
photoreceptor outer segment,photoreceptor inner segment,nucleus,cytoplasm,centrosome,axoneme,midbody,motile cilium,photoreceptor connecting cilium,mitotic spindle,ciliary tip,Expression:
Brain,
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Catalog Number
- Amount:100ul
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