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20S Proteasome α3 (phospho Ser250) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60396

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250. AA range:206-255

  • Source:

    Polyclonal, Rabbit

  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Gene Name:

    PBOV1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    proteasome subunit alpha 3(PSMA3) Homo sapiens The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Proteasome subunit alpha type-3

  • Human Gene Id:

    5684

  • Human Swiss Prot No:

    P25P88

  • Specificity:

    Phospho-20S Proteasome α3 (S250) Polyclonal Antibody detects endogenous levels of 20S Proteasome α3 protein only when phosphorylated at S250.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PSMA3; HC8; PSC8; Proteasome subunit alpha type-3; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8

  • MolecularWeight(Da):

    28433

  • Observed Band(KD):

    32

  • Function:

    catalytic activity:Cleavage of peptide bonds with very broad specificity.,function:The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.,similarity:Belongs to the peptidase T1A family.,subunit:The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.,subunit:The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are

  • Subcellular Location:

    proteasome complex,nucleus,nucleoplasm,cytoplasm,cytosol,proteasome core complex,proteasome core complex, alpha-subunit complex,extracellular exosome,

  • Expression:

    Bone marrow,Epithelium,Liver,Pancreas,Urinary bladder,

  • Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using Proteasome alpha3 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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20S Proteasome α3 (phospho Ser250) Polyclonal Antibody | EnoGene Biotech Co. Ltd