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NIPA (phospho Ser354) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60385

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NIPA around the phosphorylation site of Ser354. AA range:320-369

  • Gene Name:

    ZC3HC1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using NIPA (Phospho-Ser354) Antibody. The lane on the right is blocked with the phospho peptide.

  • Background:

    zinc finger C3HC-type containing 1(ZC3HC1) Homo sapiens This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013],

  • Protein Name:

    Nuclear-interacting partner of ALK

  • Human Gene Id:

    51530

  • Human Swiss Prot No:

    Q86WB0

  • Specificity:

    Phospho-NIPA (S354) Polyclonal Antibody detects endogenous levels of NIPA protein only when phosphorylated at S354.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ZC3HC1; NIPA; HSPC216; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1

  • MolecularWeight(Da):

    55262

  • Observed Band(KD):

    55

  • Function:

    caution:Reported to contain a F-box domain (PubMed:16009132). Such domain is however not predicted by any detection method.,developmental stage:Weakly expressed in G0/G1 phases, abundant during S and G2/M phases, and strongly decreases thereafter.,domain:The F-box-like region is required for the interaction with SKP1A.,function:Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated.

  • Subcellular Location:

    nucleus,nuclear membrane,

  • Expression:

    Epithelium,Eye,Kidney,Mammary gland,Umbilical cord blood,

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Catalog Number

  • Amount:100ul
  • Price:225$

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NIPA (phospho Ser354) Polyclonal Antibody | EnoGene Biotech Co. Ltd