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NOS3 (phospho Ser615) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60381

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser615. AA range:581-630

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    NOS3

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eNOS (Phospho-Ser615) Antibody

  • Background:

    nitric oxide synthase 3(NOS3) Homo sapiens Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

  • Protein Name:

    Nitric oxide synthase endothelial

  • Human Gene Id:

    4846

  • Human Swiss Prot No:

    P29474

  • Mouse Gene Id:

    18127

  • Mouse Swiss Prot No:

    P70313

  • Rat Gene Id:

    24600

  • Rat Swiss Prot No:

    Q62600

  • Specificity:

    Phospho-NOS3 (S615) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S615.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

  • MolecularWeight(Da):

    133289

  • Observed Band(KD):

    130

  • Function:

    catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like

  • Subcellular Location:

    Golgi membrane,nucleus,cytoplasm,cytosol,cytoskeleton,plasma membrane,caveola,endocytic vesicle membrane,

  • Expression:

    Artery,Placenta,Platelet,Umbilical vein,

  • Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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NOS3 (phospho Ser615) Polyclonal Antibody | EnoGene Biotech Co. Ltd