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NFκB-p100 (phospho Ser872) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60375

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NF-kappaB p100 around the phosphorylation site of Ser872. AA range:838-887

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    NFKB2

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

  • Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 (Phospho-Ser872) Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(Immunoway cat:RS23920)was diluted at 1:10000, 37° 1hour.

  • Background:

    nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

  • Protein Name:

    Nuclear factor NF-kappa-B p100 subunit

  • Human Gene Id:

    4791

  • Human Swiss Prot No:

    Q00653

  • Mouse Gene Id:

    18034

  • Mouse Swiss Prot No:

    Q9WTK5

  • Specificity:

    Phospho-NFκB-p100 (S872) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S872.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10

  • MolecularWeight(Da):

    96749

  • Observed Band(KD):

    96

  • Function:

    disease:A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.,disease:A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT).,disease:In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.,domain:The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation.,domain:The glycine-rich region (GRR) appears to be a critical element in the generation of p52.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost a

  • Subcellular Location:

    nucleus,nucleoplasm,cytoplasm,cytosol,Bcl3/NF-kappaB2 complex,

  • Expression:

    Leukemia,Lymph,Thymus,

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Catalog Number

  • Amount:100ul
  • Price:225$

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