Cdk9 (phospho Thr186) Polyclonal Antibody

Name: Cdk9 (phospho Thr186) Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-60365
Price: 225 USD
Availability: InStock

E20-60365Cite ID: EN0049291Datasheet COA MSDS

ReactivityHuman;Mouse;Rat

ApplicationsWB;ELISA

SourcePolyclonal, Rabbit,IgG

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (1)Documents

Product Information

Molecular Weight(Da):42778

Protein NameCyclin-dependent kinase 9

Human Gene Id1025

Human Swiss Prot NoP50750

Mouse Gene Id107951

Mouse Swiss Prot NoQ99J95

Rat Gene Id362110

Rat Swiss Prot NoQ641Z4

SpecificityPhospho-Cdk9 (T186) Polyclonal Antibody detects endogenous levels of Cdk9 protein only when phosphorylated at T186.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameCDK9; CDC2L4; TAK; Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit

Observed Band(KD)42

Functioncatalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Associates with CCNT1/cyclin-T1 to form P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Also associates with CCNK/cyclin-K.

Subcellular LocationNucleus. Cytoplasm. Nucleus, PML body. Accumulates on chromatin in response to replication stress. Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated.

ExpressionUbiquitous.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186. AA range:152-201

Gene NameCDK9

DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Backgroundcyclin dependent kinase 9(CDK9) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-60365-Conjugate. Price: $485 / Size: 100μl.

E20-60365-FITC$485 / 100μlE20-60365-Biotin$485 / 100μlE20-60365-HRP$485 / 100μlE20-60365-AP$485 / 100μlE20-60365-AF350$485 / 100μlE20-60365-AF405M$485 / 100μlE20-60365-AF488$485 / 100μlE20-60365-AF514$485 / 100μlE20-60365-AF532$485 / 100μlE20-60365-AF555$485 / 100μlE20-60365-AF568$485 / 100μlE20-60365-AF405S$485 / 100μlE20-60365-AF405L$485 / 100μlE20-60365-AF546$485 / 100μlE20-60365-AF594$485 / 100μlE20-60365-AF610$485 / 100μlE20-60365-AF635$485 / 100μlE20-60365-AF647$485 / 100μlE20-60365-AF680$485 / 100μlE20-60365-AF700$485 / 100μlE20-60365-AF750$485 / 100μlE20-60365-AF790$485 / 100μlE20-60365-APC$485 / 100μlE20-60365-PE$485 / 100μlE20-60365-Cy3$485 / 100μlE20-60365-Cy5$485 / 100μlE20-60365-Cy5.5$485 / 100μlE20-60365-Cy7$485 / 100μlE20-60365-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.