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MCM4 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60354

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    MCM4

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Background:

    minichromosome maintenance complex component 4(MCM4) Homo sapiens The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri

  • Protein Name:

    DNA replication licensing factor MCM4

  • Human Gene Id:

    4173

  • Human Swiss Prot No:

    P33991

  • Mouse Gene Id:

    17217

  • Mouse Swiss Prot No:

    P49717

  • Specificity:

    Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21

  • MolecularWeight(Da):

    96558

  • Observed Band(KD):

    85

  • Function:

    function:Involved in the control of DNA replication.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,

  • Subcellular Location:

    nuclear chromosome, telomeric region,nucleus,nucleoplasm,membrane,MCM complex,

  • Expression:

    Cervix,Epithelium,Testis,

  • Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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MCM4 Polyclonal Antibody | EnoGene Biotech Co. Ltd