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Raf-1 (phospho Ser43) Polyclonal Antibody
Datasheet
Catalog Number:
E20-60353Amount:
100ulSwiss-Prot No.:
P04049/ Q99N57/P11345Molecular Weight:
73052，Observed Band : 73kdReactivity:
Human,Mouse,RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogenImmunogen:
Synthesized phospho-peptide around the phosphorylation site of human Raf-1
(phospho Ser301)Specificity/Sensitivity:
Phospho-Raf-1 (S43) Polyclonal Antibody detects endogenous levels of Raf-1
protein only when phosphorylated at S43.Storage/Stability:
-20°C/1 yearSource:
RabbitGene Name:
RAF1Dilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other
- Western blot analysis of lysates from NIH/3T3 cells, using C-RAF (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.
Background:
Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene
is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP
kinase kinase kinase (MAP3K), which functions downstream of the Ras family of
membrane associated GTPases to which it binds directly. Once activated, the
cellular RAF1 protein can phosphorylate to activate the dual specificity protein
kinases MEK1 and MEK2, which in turn phosphorylate to activate the
serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are
pleiotropic effectors of cell physiology and play an important role in the control of
gene expression involved in the cell division cycle, apoptosis, cell differentiation
and cell migration. Mutations in this gene are associated with Noonan syndrome 5
and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],Gene ID:
5894/110157/24703Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azideFunction:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2
zinc ions per subunit.,disease:Defects in RAF1 are the cause of LEOPARD
syndrome type 2 (LEOPARD syndrome-2) [MIM:611554]. LEOPARD syndrome is
an autosomal dominant disorder allelic with Noonan syndrome. The acronym
LEOPARD stands for lentigines, electrocardiographic conduction abnormalities,
ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of
growth, and deafness.,disease:Defects in RAF1 are the cause of Noonan
syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder
characterized by dysmorphic facial features, short stature, hypertelorism, cardiac
anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically
heterogeneous and relatively common syndrome, with an estimated incidence of
1 in 1000-2500 live births.,function:Involved in the transducti
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