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PKD2 (phospho Ser876) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60341

  • Amount:

    100ul

  • Molecular Weight:

    96722Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PKD2 around the phosphorylation site of Ser876. AA range:829-878

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of HELA cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    PRKD2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Serine/threonine-protein kinase D2

  • Human Gene Id:

    25865

  • Human Swiss Prot No:

    Q9BZL6

  • Mouse Gene Id:

    101540

  • Mouse Swiss Prot No:

    Q8BZ03

  • Rat Gene Id:

    292658

  • Rat Swiss Prot No:

    Q5XIS9

  • Specificity:

    Phospho-PKD2 (S876) Polyclonal Antibody detects endogenous levels of PKD2 protein only when phosphorylated at S876.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2

  • Observed Band(KD):

    96

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed.,

  • Subcellular Location:

    Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. .

  • Expression:

    Widely expressed.

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