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PKD1 (phospho Ser910) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60332

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu around the phosphorylation site of Ser910. AA range:863-912

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of A431 cells using Phospho-PKD1 (S910) Polyclonal Antibody

  • Gene Name:

    PRKD1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    protein kinase D1(PRKD1) Homo sapiens PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],

  • Protein Name:

    Serine/threonine-protein kinase D1

  • Human Gene Id:

    5587

  • Human Swiss Prot No:

    Q15139

  • Specificity:

    Phospho-PKD1 (S910) Polyclonal Antibody detects endogenous levels of PKD1 protein only when phosphorylated at S910.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu

  • MolecularWeight(Da):

    101888

  • Observed Band(KD):

    117

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase involved in resistance to oxidative stress.,PTM:Phosphorylation of Ser-738 and/or Ser-742 in activated PKD is mediated by transphosphorylation (By similarity). Phosphorylation of Tyr-463 mediated by the Src/Abl pathway in response to oxidative stress activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with Src.,

  • Subcellular Location:

    intracellular,nucleus,cytoplasm,Golgi apparatus,trans-Golgi network,cytosol,plasma membrane,integral component of plasma membrane,cell-cell junction,cell cortex,

  • Expression:

    Placenta,Testis,

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD1/PKC mu (Phospho-Ser910) Antibody

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Catalog Number

  • Amount:100ul
  • Price:225$

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PKD1 (phospho Ser910) Polyclonal Antibody | EnoGene Biotech Co. Ltd