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Pim-1 (phospho Tyr309) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60331

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Pim-1 around the phosphorylation site of Tyr309. AA range:281-330

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PIM1

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

  • Wang, T., Liang, L., Zhao, C. et al. Elucidating direct kinase targets of compound Danshen dropping pills employing archived data and prediction models. Sci Rep 11, 9541 (2021).

  • Background:

    Pim-1 proto-oncogene, serine/threonine kinase(PIM1) Homo sapiens The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011],

  • Protein Name:

    Serine/threonine-protein kinase pim-1

  • Human Gene Id:

    5292

  • Human Swiss Prot No:

    P11309

  • Mouse Swiss Prot No:

    P06803

  • Rat Gene Id:

    24649

  • Rat Swiss Prot No:

    P26794

  • Specificity:

    Phospho-Pim-1 (Y309) Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Y309.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PIM1; Serine/threonine-protein kinase pim-1

  • MolecularWeight(Da):

    45412

  • Observed Band(KD):

    45

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Manganese.,function:Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.,induction:Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines.,PTM:Autophosphorylated on both serine/threonine and tyrosine residues.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.,tissue specificity:Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 an

  • Subcellular Location:

    nucleus,cytoplasm,plasma membrane,

  • Expression:

    Kidney,

  • Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Pim-1 (phospho Tyr309) Polyclonal Antibody | EnoGene Biotech Co. Ltd