HDAC5/9 (phospho Ser259/220) Polyclonal Antibody

Name: HDAC5/9 (phospho Ser259/220) Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-60299
Price: 225 USD
Availability: InStock

E20-60299Cite ID: EN0049225Datasheet COA MSDS

ReactivityHuman;Mouse

ApplicationsWB;IHC-p;IF(paraffin section);ELISA

SourcePolyclonal, Rabbit,IgG

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight121992Da

Protein NameHistone deacetylase 5/9

Human Gene Id10014/9734

Human Swiss Prot NoQ9UQL6/Q9UKV0

Mouse Gene Id79221

SpecificityPhospho-HDAC5/9 (S259/220) Polyclonal Antibody detects endogenous levels of HDAC5/9 protein only when phosphorylated at S259/220.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameHDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription rep

Observed Band(KD)121

Functioncatalytic activity:Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.,domain:The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.,function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.,PTM:Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm.,PTM:

Subcellular LocationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

ExpressionUbiquitous.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser259. AA range:225-274

Gene NameHDAC5/HDAC9

DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

BackgroundHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-60299-Conjugate. Price: $485 / Size: 100μl.

E20-60299-FITC$485 / 100μlE20-60299-Biotin$485 / 100μlE20-60299-HRP$485 / 100μlE20-60299-AP$485 / 100μlE20-60299-AF350$485 / 100μlE20-60299-AF405M$485 / 100μlE20-60299-AF488$485 / 100μlE20-60299-AF514$485 / 100μlE20-60299-AF532$485 / 100μlE20-60299-AF555$485 / 100μlE20-60299-AF568$485 / 100μlE20-60299-AF405S$485 / 100μlE20-60299-AF405L$485 / 100μlE20-60299-AF546$485 / 100μlE20-60299-AF594$485 / 100μlE20-60299-AF610$485 / 100μlE20-60299-AF635$485 / 100μlE20-60299-AF647$485 / 100μlE20-60299-AF680$485 / 100μlE20-60299-AF700$485 / 100μlE20-60299-AF750$485 / 100μlE20-60299-AF790$485 / 100μlE20-60299-APC$485 / 100μlE20-60299-PE$485 / 100μlE20-60299-Cy3$485 / 100μlE20-60299-Cy5$485 / 100μlE20-60299-Cy5.5$485 / 100μlE20-60299-Cy7$485 / 100μlE20-60299-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.