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E2F-1 (phospho Thr433) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60298

  • Amount:

    100ul

  • Molecular Weight:

    46920Da

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433. AA range:388-437

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    E2F1

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

  • Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.

  • Protein Name:

    Transcription factor E2F1

  • Human Gene Id:

    1869

  • Human Swiss Prot No:

    Q01094

  • Specificity:

    Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1

  • Observed Band(KD):

    70

  • Background:

    E2F transcription factor 1(E2F1) Homo sapiens The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media

  • Function:

    function:Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, ren

  • Subcellular Location:

    nuclear chromatin,nucleus,nucleoplasm,mitochondrion,Rb-E2F complex,

  • Expression:

    Brain,Epithelium,Pancreas,Skin,

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Catalog Number

  • Amount:100ul
  • Price:225$

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E2F-1 (phospho Thr433) Polyclonal Antibody | EnoGene Biotech Co. Ltd