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Cyclin D1 (phospho Thr286) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60297

  • Amount:

    100ul

  • Molecular Weight:

    33729Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286. AA range:246-295

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500

  • Gene Name:

    CCND1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008],

  • Protein Name:

    G1/S-specific cyclin-D1

  • Human Gene Id:

    595

  • Human Swiss Prot No:

    P24385

  • Mouse Gene Id:

    12443

  • Mouse Swiss Prot No:

    P25322

  • Rat Gene Id:

    58919

  • Rat Swiss Prot No:

    P39948

  • Specificity:

    Phospho-Cyclin D1 (T286) Polyclonal Antibody detects endogenous levels of Cyclin D1 protein only when phosphorylated at T286.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene

  • Observed Band(KD):

    33

  • Function:

    disease:A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.,disease:A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.,disease:A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.,function:Essential for the control of the cell cycle at the G1/S (start) transition.,online information:The Singapore human mutation and polymorphism database,PTM:Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31.

  • Subcellular Location:

    Nucleus . Cytoplasm . Nucleus membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. .

  • Expression:

    Brain,Placenta,Tongue,

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