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Btk (phospho Tyr223) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60287

  • Amount:

    100ul

  • Molecular Weight:

    76150Da

  • Applications:

    WB;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223. AA range:188-237

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Btk (Y223) Polyclonal Antibody

  • Gene Name:

    BTK

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013],

  • Protein Name:

    Tyrosine-protein kinase BTK

  • Human Gene Id:

    695

  • Human Swiss Prot No:

    Q06187

  • Mouse Gene Id:

    12229

  • Mouse Swiss Prot No:

    P35991

  • Specificity:

    Phospho-Btk (Y223) Polyclonal Antibody detects endogenous levels of Btk protein only when phosphorylated at Y223.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    BTK; AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase

  • Observed Band(KD):

    80

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,disease:Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also called X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.,

  • Subcellular Location:

    Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.

  • Expression:

    Predominantly expressed in B-lymphocytes.

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Catalog Number

  • Amount:100ul
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Btk (phospho Tyr223) Polyclonal Antibody | EnoGene Biotech Co. Ltd