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Sp1 (phospho Thr739) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60248

  • Amount:

    100ul

  • Molecular Weight:

    80693Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr739. AA range:706-755

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Sp1 (T739) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    SP1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014],

  • Protein Name:

    Transcription factor Sp1

  • Human Gene Id:

    6667

  • Human Swiss Prot No:

    P08047

  • Mouse Gene Id:

    20683

  • Mouse Swiss Prot No:

    O89090

  • Rat Gene Id:

    24790

  • Rat Swiss Prot No:

    Q01714

  • Specificity:

    Phospho-Sp1 (T739) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T739.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    SP1; TSFP1; Transcription factor Sp1

  • Observed Band(KD):

    81

  • Function:

    function:Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.,PTM:O-glycosylated; contains N-acetylglucosamine side chains.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding.,

  • Subcellular Location:

    Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.

  • Expression:

    Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.

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Catalog Number

  • Amount:100ul
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Sp1 (phospho Thr739) Polyclonal Antibody | EnoGene Biotech Co. Ltd