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PKC ζ (phospho Thr560) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60230

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr560. AA range:526-575

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    PRKCZ

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    protein kinase C zeta(PRKCZ) Homo sapiens Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Protein kinase C zeta type

  • Human Gene Id:

    5590

  • Human Swiss Prot No:

    Q05513

  • Specificity:

    Phospho-PKC ζ (T560) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T560.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

  • MolecularWeight(Da):

    67660

  • Observed Band(KD):

    80

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,domain:The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.,enzyme regulation:Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator. Two specific sites, Thr-410 (activation loop of the kinase domain) and Thr-560 (turn motif), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to

  • Subcellular Location:

    intracellular,nuclear envelope,cytoplasm,endosome,microtubule organizing center,cytosol,plasma membrane,cell-cell junction,bicellular tight junction,membrane,apical plasma membrane,nuclear matrix,cell junction,

  • Expression:

    Brain,Colon,Epithelium,Frontal cortex,Hippocampus,Neuroblastoma,Ovary,

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody

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Catalog Number

  • Amount:100ul
  • Price:225$

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PKC ζ (phospho Thr560) Polyclonal Antibody | EnoGene Biotech Co. Ltd