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PI 3-kinase p85β (phospho Tyr464) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60225

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized phospho-peptide around the phosphorylation site of human PI 3-kinase p85β (phospho Tyr464)

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PIK3R2

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PI 3-kinase p85β (Phospho-Tyr464) Antibody

  • Background:

    phosphoinositide-3-kinase regulatory subunit 2(PIK3R2) Homo sapiens Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012],

  • Protein Name:

    Phosphatidylinositol 3-kinase regulatory subunit beta

  • Human Gene Id:

    5296

  • Human Swiss Prot No:

    O00459

  • Mouse Gene Id:

    18709

  • Mouse Swiss Prot No:

    O08908

  • Rat Swiss Prot No:

    Q63788

  • Specificity:

    Phospho-PI 3-kinase p85β (Y464) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85β protein only when phosphorylated at Y464.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PIK3R2; Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kina

  • MolecularWeight(Da):

    81624

  • Observed Band(KD):

    85

  • Function:

    function:Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits.,

  • Subcellular Location:

    nucleus,cytosol,phosphatidylinositol 3-kinase complex,

  • Expression:

    Brain,Epithelium,Kidney,Placenta,

  • Western blot analysis of lysate from Jurkat cells, using phospho-PI 3-kinase p85β (Phospho-Tyr464) antibody.

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Catalog Number

  • Amount:100ul
  • Price:225$

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PI 3-kinase p85β (phospho Tyr464) Polyclonal Antibody | EnoGene Biotech Co. Ltd