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PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60224

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199. AA range:436-485

  • Source:

    Polyclonal, Rabbit

  • Yang, Yazong, et al. "Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells." Oncotarget 7.25 (2016): 38025.

  • Gene Name:

    PIK3R1/PIK3R3

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    phosphoinositide-3-kinase regulatory subunit 1(PIK3R1) Homo sapiens Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],

  • Protein Name:

    Phosphatidylinositol 3-kinase regulatory subunit alpha/gamma

  • Human Gene Id:

    5295

  • Human Swiss Prot No:

    P27986/Q92569

  • Mouse Gene Id:

    18708/18710

  • Rat Gene Id:

    25513/60664

  • Rat Swiss Prot No:

    Q63787/Q63789

  • Specificity:

    Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

  • MolecularWeight(Da):

    83598/54462

  • Observed Band(KD):

    55+85

  • Function:

    disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat

  • Subcellular Location:

    nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,

  • Expression:

    Brain,Epithelium,Lung,Placenta,Skeletal muscle,

  • Immunofluorescence analysis of human-lung tissue. 1,PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Catalog Number

  • Amount:100ul
  • Price:225$

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PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody | EnoGene Biotech Co. Ltd